tissue homogenization is the process of
breaking down tissue structure to form a
suspension of tissue
solids proteins and fluids
remove the organ or apart from the
animal carefully and cut into into small
pieces
with the help of forces
put one by one piece into a solution
actually a one percent solution is
advised to be prepared in suitable
buffer
as per the protocol
in this process
this porter album type homogenizer with
a glass tube are used for
homogenizer so it is a glass tube
where the solution has to be repaired
and
then there is one shaft
put the tube into the
the shaft is inserted into the tube
and
slowly
the speed is increase of the
homogenization
many times it has been observed that if
you increase a speed immediately then
there is a chances of breakdown of the
apparatus
all the process should be carried out
under
chilled environment
because
we are going to detect the enzyme into
the homogeneity as the temperature it
increases
it will destruct the enzyme activity
again the homogenization can develop the
friction which can also increases
the temperature of the solution
once the solution is formed
stop the process and
centrifuge
the solution the homogeneity you will
get
supernatant which is a clear solution
and another is the sediment which is a
membrane bound portion which is remain
at the end
this both portion can be used for the
estimation of
various enzymes
as per the protocol or as per the
required
